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美国戴安Dionex MAbPac SEC-1 analytical column 075592 生物色谱柱

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产品名称: 美国戴安Dionex MAbPac SEC-1 analytical column 075592 生物色谱柱
产品型号: 075592
产品展商: 美国戴安Dionex
产品文档: 无相关文档

简单介绍

戴安公司生产的仪器主要有离子色谱仪、高效液相色谱仪、快速溶剂萃取仪和氨基酸直接分析仪。其中离子色谱仪凭借着雄厚的技术优势和方法已广泛地应用于各个领域,其市场占有率达80%。全球与离子色谱相关的文章有90%均出自戴安公司生产的离子色谱仪。   戴安公司的快速溶剂萃取仪15分钟之内仅用15毫升溶剂既可完成对10g样品的萃取,该技术已在环境、药物、食品和聚合物工业等领域得到广泛应用。


美国戴安Dionex MAbPac SEC-1 analytical column 075592 生物色谱柱  的详细介绍

MAbPac SEC– 1 for Monoclonal Antibody (MAb) Analysis

MAbPac SEC-1 is a size exclusion chromatography (SEC) column designed for monoclonal antibody (MAb) analysis, including monomers, aggregates, and fragments, under non-denaturing conditions, and in both high- and low-salt mobile phases and volatile eluents.

• Proprietary hydrophilic bonded layer results in minimal non-desired interactions between the biomolecules and the stationary phase.
• Nonmetallic and Biocompatible PEEK housing eliminates metal contamination from the column hardware.
• Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and Corona® CAD detection.
• Rugged, reproducible column.
• Superior performance for the analysis of monoclonal antibodies, even at low salt concentrations.


Applications
SEC is frequently used because of its simple operating principle and features not found in other separation techniques including separating aggregates that are not easily distinguished by other chromatographic means. MAbs produced from mammalian cell culture may contain significant amounts of dimers and higher-order aggregates. The MAbPac SEC-10 is a high-resolution SEC column that is suitable for use with a wide range of biomolecules, including monoclonal antibodies and their aggregates.  

MAbPac SEC-1 Monoclonal Antibody Analysis Column Specifications
Column Dimension 4 × 300 mm, 4 × 50 mm, PEEK housing
Particle Size 5 μm
Bonding Chemistry Proprietary diol
Silica Substrate Spherical, high-purity porous silica
Pore size 300 Å
Flow Rate Range: 0.2 to 0.3 mL/min.
Pressure Limit 1800 psi
Temperature Range: Ambient to 25 ºC
pH Range 2.2–7.5
Typical buffers:

50 mM phosphate buffer (pH 6.8) + 0.3 M NaCl, 

PBS and other commonly used buffers

Solvent Compatibility: Compatible with 100% organic solvents
Separation range for globular proteins 10,000–1,000,000
Exclusion limit for globular proteins >1,000,000
Detergent Compatibility

Compatible with 0.1% SDS (though binding will be irreversible and

it is recommended that the column be dedicated to this application)


The MAbPac SEC-1 column is based on high-purity, spherical, porous (300 Å), 5 µm silica particles covalently modified with a proprietary diol hydrophilic layer. The column is packed into nonmetallic, biocompatible, PEEK column housing to eliminate compromised chromatographic due to metal from the column hardware.

Analytical Columns
MAbPac SEC-1, 5µm, 300Å, analytical column PEEK (4.0 x 300mm) 074696
MAbPac SEC-1, 5µm, 300Å, analytical column PEEK (4.0 x 150mm) 075592
Guard Columns
MAbPac SEC-1, 5µm, 300Å, guard column PEEK (4.0 x 50mm) 074697


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