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074600美国戴安Dionex ProPac SCX and WCX Protein Column 生物色谱柱

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产品名称: 074600美国戴安Dionex ProPac SCX and WCX Protein Column 生物色谱柱
产品型号: 074600
产品展商: 美国戴安Dionex
产品文档: 无相关文档

简单介绍

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074600美国戴安Dionex ProPac SCX and WCX Protein Column 生物色谱柱  的详细介绍

Strong and Weak Cation-Exchange Columns

for High-Resolution Separation of Proteins and MAbs

New! The Thermo Scientific ProPac SCX column line has expanded to include the next generation ProPac SCX-20 column. This column provides unequalled high resolution of protein variants.

Please also see the Thermo Scientific MAbPac SCX-10 column, a strong cation-exchange column designed specifically for high-resolution, high-efficiency analysis of monoclonal antibodies (MAbs) and associated variants. This column is complimentary to the industry-leading ProPac WCX-10 column for monoclonal antibody variant analysis. The MAbPac SCX-10 offers an alternative selectivity and provides higher resolution and efficiency for variant analysis of most monoclonal antibody samples than the ProPac WCX-10 column.

ProPac SCX-10 and WCX-10 strong and weak cation-exchange columns are based on a nonporous core particle that results in high-efficiency peaks and high-resolution separations of protein variants. A hydrophilic layer prevents unwanted secondary interactions. A grafted cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

  • Characterization and quality control assessment of monoclonal antibodies and other proteins
  • Unequalled resolution
  • High-resolution separations with excellent sample recovery
  • Unmatched column-to-column and lot-to-lot reproducibility
  • Useful for characterization of related protein variants (e.g., deamidation and MAb lysine truncation variants)
  • Highly-efficient peaks with excellent sample recovery
  • Available in different dimensions and formats for flexibility in method development

The ProPac SCX-10 is a strong cation-exchange column with sulfonate functional groups, and the ProPac WCX-10 column has a carboxylate functional group. Both are based on 10 µm nonporous, polymeric beads and coated with a proprietary hydrophilic layer.

All ProPac columns are manufactured and tested under the strictest specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. These columns are available as lot-select packages containing three columns from a single lot or three columns from three different lots, to assist quality control scientists in method validation.

ProPac SCX-10 Strong Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms  Specifications
Maximum operating pressure 3000 psi (21 MPa)
Maximum operating temperature 60 °C
Recommended operating temperature: <40 °C
Typical operating conditions UV detection at 1 mL/min
Recommended flow rate: 1 mL/min
Mobile phase compatibility pH 2–12; 80% compatible with acetonitrile, acetone, methanol.
Compatible with nonionic and zwitterionic detergents.
Incompatible with cationic detergents.
Resin composition 10-µm-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the sulfonate functional groups.
Anion-exchange capacity < 100 µg protein on-column, depending upon the protein
(4 × 250 mm analytical column)
Column construction PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
ProPac WCX-10 Weak Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms  Specifications
Resin composition 10-µm-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the carboxylate functional groups.
Anion-exchange capacity < 100 µg protein on-column, depending upon the protein
(4 × 250 mm analytical column)
Column construction PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
Maximum operating pressure 3000 psi (21 MPa)
Maximum operating temperature 60 °C
Recommended operating temperature: <40 °C
Typical operating conditions UV detection at 1 mL/min
Recommended flow rate: 1 mL/min
Mobile phase compatibility pH 2–12; 80% compatible with acetonitrile and acetone.
Compatible with nonionic and zwitterionic detergents.
Incompatible with cationic detergents.
SCX Analytical Columns
ProPac SCX-20 Analytical Column (4 x 250 mm) 074628
ProPac SCX-10 Analytical Column (4 x 250 mm) 054995
ProPac SCX-10 Analytical Column (2 x 250 mm) 063456
ProPac SCX-10 Semi-Preparative Column (9 x 250 mm) 063700
ProPac SCX-10 Semi-Preparative Column (22 x 250 mm) SP5522
ProPac SCX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin) SP5727
ProPac SCX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin) SP5728
SCX Guard Columns
ProPac SCX-20 Guard Column (4 x 50mm) 074643
ProPac SCX-10G Guard Column (4 x 50 mm) 079930
ProPac SCX-10G Guard Column (2 x 50 mm) 063462
WCX Analytical Columns
ProPac WCX-10HT Analytical Column (4x 50mm) 074600
ProPac WCX-10 Analytical Column (4 x 100 mm) SP5829
ProPac WCX-10 Analytical Column (4 x 150 mm) SP6703
ProPac WCX-10 Analytical Column (4 x 250 mm) 054993
ProPac WCX-10 Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin) SP5512
ProPac WCX-10 Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin) SP5513
ProPac WCX-10 Analytical Column (2 x 250 mm) 063472
ProPac WCX-10 Semi-Preparative Column (9 x 250 mm) 063474
ProPac WCX-10 Semi-Preparative Column (22 x 250 mm) SP5482
WCX Guard Columns
ProPac WCX-10G Guard Column (2 x 50 mm) 063480
ProPac WCX-10G Guard Column (4 x 50 mm) 054994

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