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SP2667美国戴安Dionex CarboPac PA100 Carbohydrate Column 生物色谱柱

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产品名称: SP2667美国戴安Dionex CarboPac PA100 Carbohydrate Column 生物色谱柱
产品型号: SP2667
产品展商: 美国戴安Dionex
产品文档: 无相关文档

简单介绍

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SP2667美国戴安Dionex CarboPac PA100 Carbohydrate Column 生物色谱柱  的详细介绍

Low-Picomole Oligosaccharide Analysis

Oligosaccharides released from therapeutic glycoproteins can differ in size, charge, branching, and linkage. Oligosaccharides with these features can be resolved using the CarboPac® PA100. It provides the flexibility to separate oligosaccharides into broad classes depending upon the degree of sialylation, while maintaining the resolution needed for routine analysis based on linkage isomerism. Coupled with pulsed amperometric detection, the PA100 supports detection at low-picomole levels.

  • Separation of closely related oligosaccharides, even isomers, at picomole levels
  • Separation of neutral and charged oligosaccharides in the same run
  • No sample derivatization required for detection

The CarboPac PA100 column is a high-resolution, strong anion-exchange column for enhanced chromatography of oligosaccharides.

The Carbohydrate Membrane Desalter can be used on-line prior to fraction collection.

CarboPac PA100 Column for Oligosaccharide Analysis Specifications
Dimensions CarboPac PA100 Analytical Column:
4 × 250 mm
CarboPac PA100 Guard Column:
4 × 50 mm
CarboPac PA100 Microbore Column:
2 × 250 mm
CarboPac PA100 Microbore Guard Column:
2 × 50 mm
CarboPac PA100 Semipreparative Column:
9 × 250 mm
CarboPac PA100 Semipreparative Column:
22 × 250 mm
Maximum operating pressure 4000 psi (27.9 MPa)
Temperature range 4–60 °C
Recommended operating temperature: ambient
Typical operating conditions 2400 psi at 1.0 mL/min (analytical and guard columns)
Recommended flow rate: 1.0 mL/min
Mobile phase compatibility pH 0–14; 100% compatible with common organic solvents.
Ionic form eluents: sodium acetate and sodium hydroxide only.
Resin composition 8.5-µm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linking) agglomerated with 275-nm MicroBead quaternary amine functionalized latex (6% cross-linked).
Anion-exchange capacity Approximately 90 µeq/column (4 × 250 mm analytical column)
Column construction PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
Analytical Columns
CarboPac PA100 Analytical Column (2 x 250 mm) 057182
CarboPac PA100 Analytical Column (4 x 250 mm) 043055
CarboPac PA100 Column (9 x 250 mm) SP2089
CarboPac PA100 Column (22 x 250 mm) SP2667
Guard Columns
CarboPac PA100 Guard Column (2 x 50 mm) 057183
CarboPac PA100 Guard Column (4 x 50 mm) 043054

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