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美国戴安Dionex PepSwift Columns 164585 生物色谱柱

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产品名称: 美国戴安Dionex PepSwift Columns 164585 生物色谱柱
产品型号: 164585
产品展商: 美国戴安Dionex
产品文档: 无相关文档

简单介绍

戴安公司生产的仪器主要有离子色谱仪、高效液相色谱仪、快速溶剂萃取仪和氨基酸直接分析仪。其中离子色谱仪凭借着雄厚的技术优势和方法已广泛地应用于各个领域,其市场占有率达80%。全球与离子色谱相关的文章有90%均出自戴安公司生产的离子色谱仪。   戴安公司的快速溶剂萃取仪15分钟之内仅用15毫升溶剂既可完成对10g样品的萃取,该技术已在环境、**、食品和聚合物工业等领域得到广泛应用。


美国戴安Dionex PepSwift Columns 164585 生物色谱柱  的详细介绍

PepSwift Columns

Monolithic Columns for Fast, High-Resolution Protein and Peptide Analysis

PepSwift monolithic columns are specially designed for fast separation and identification of proteins and peptides using nano and capillary LC coupled to MS. PepSwift columns are based on a polystyrene divinylbenzene copolymer bed. The bed structure of the column offers a high-quality alternative to traditional microparticulate sorbents, providing important advantages for high-sensitivity proteomics applications.

  • Polymeric monolithic stationary phases
  • High-speed protein/peptide separations (< 10 min)
  • High separation efficiency (> 200.000 plates/m)
  • High column-to-column reproducibility
  • High sensitivity in LC/MS
  • Superior lifetime
  • Available in 100 μm, 200 μm and 500 μm i.d 
  • Available in 5 and 25 cm length (only 100 and 200 µm i.d.)
  • nanoViper fitting

PepSwift Trap columns can be used for preconcentration and desalting of samples consisting of peptides and proteins without negative impact on the chromatographic performance or recovery of the compounds. Various ion-pairing agents can be used in the loading solvent and/or mobile phases to change the selectivity of the separation or improve the trapping efficiency.

 

PepSwift and ProSwift Capillary Monolithic Reversed-Phase Columns Specifications
 
PepSwift RP
Typical Applications Peptides, proteins, and oligonucleotides
Column Dimensions 100 μm × 50 mm 200 μm × 50 mm 500 μm × 50 mm
Base Material Polystyrene/divinyl benzene Polystyrene/divinyl benzene Polystyrene/divinyl benzene
Chemistry Phenyl Phenyl Phenyl
Recommended Flow Rate 700–1000 nL/min 2–3 μL/min 15–25 μL/min
Temperature 80 ˚C 80 ˚C 80 ˚C
Maximum Pressure 4350 psi (30 MPa) 4350 psi (30 MPa) 4350 psi (30 MPa)
Recommended Sample Quantity 5 ng* 20 ng* 0.12 μg*
Sample Capacity*** 0.2 ng 0.6 ng 3.8 ng
pH Stability 1–10 1–10 1–10
Solvent Compatibility Most common RP
solvents
Most common RP
solvents
Most common RP
solvents

*Tryptic digest of six proteins
**E. coli proteins
*** Dynamic loading capacity is defined as the 10% increase in peak width measured at half height induced by injected mass of a single peptide (tryptic peptide of cytochrome c for PepSwift RP) and intact protein (cytochrome c for ProSwift RP-10R), respectively

PepSwift and ProSwift Monolith Columns
PepSwift Monolithic Nano Column, 100 μm i.d. × 5 cm, nanoViper 164584
PepSwift Monolithic Nano Column, 100 μm i.d. × 5 cm, nanoViper 164584
PepSwift Monolithic Nano Column, 100 µm i.d. x 25 cm, nanoViper 164543
PepSwift Monolithic Capillary Column, 200 μm i.d. × 5 cm 161409
PepSwift Monolithic Capillary Column, 200 µm i.d. x 5 cm, nanoViper 164557
PepSwift Monolithic Capillary Column, 200 µm i.d. x 25 cm, nanoViper 164542
PepSwift Monolithic Capillary Column, 500 μm i.d. × 5 cm 164087
PepSwift Monolithic Capillary Column, 500 μm i.d. × 5 cm, nanoViper 164585
ProSwift RP-10R Monolithic Capillary Column, 1 mm i.d. × 5 cm, nanoViper 164586
ProSwift RP-10R Monolithic Capillary Column, 1 mm i.d. × 5 cm, nanoViper 164586
PepSwift Trap Columns
PepSwift Monolithic Trap Column, 200 µm x 5 mm, (set of 2), nanoViper 164558
PepSwift Monolithic Trap Column, 200 µm x 5 mm, (set of 2), nanoViper 164558

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